{"id":4460,"date":"2020-11-30T17:36:32","date_gmt":"2020-11-30T20:36:32","guid":{"rendered":"https:\/\/biobureau.com.br\/technological-readiness-scale-for-golden-mussel-control\/"},"modified":"2020-11-30T17:36:32","modified_gmt":"2020-11-30T20:36:32","slug":"technological-readiness-scale-for-golden-mussel-control","status":"publish","type":"post","link":"https:\/\/biobureau.com.br\/en\/technological-readiness-scale-for-golden-mussel-control\/","title":{"rendered":"Technological Readiness Scale for Golden Mussel Control"},"content":{"rendered":"

In 2017, Bio Bureau started a research program for the biotechnological control of the golden mussel, funded by China Tree Gorges – CTG Brasil, through ANEEL’s research and development program.<\/p>\n

We divided the development into stages according to the Technology Readiness Scale – TRL<\/a>. This division facilitates the understanding and communication of a project with a high degree of complexity.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Technology Readiness Level<\/td>\nTechnological goals<\/td>\n<\/tr>\n
TRL #1 Basic Principles<\/td>\n\n
TRL #2 Conceptual model<\/td>\n\n
TRL #3 Proof of concept<\/td>\n\n
    \n
  • Identification of haplosufficient somatic female fertility genes in the genome of Limnoperna fortunei<\/li>\n
  • Computer simulations of the dynamics of homing endonuclease genes (HEG) together with musseldemo<\/li>\n
  • Construction of CRISPR-Cas9 gene delivery vectors targeting female fertility genes<\/li>\n
  • Genetic transformation of mussel cells.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL #4 Lab-scale prototype<\/td>\n\n
    \n
  • Generation of genetically modified mussels with the CRISPR-Cas9 allele<\/li>\n
  • Genotyping, phenotyping and demonstration of haplosufficiency<\/li>\n
  • Laboratory testing of GM mussels in experimental aquariums under highly controlled conditions according to phase 1 of the World Health Organization (WHO) guidelines<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL #5 Field-scale prototype<\/td>\n\n
    \n
  • Breeding GM mussels with wild-type specimens in controlled mesocosm experiments<\/li>\n
  • Empirical evaluation of homing rate and CRISPR homing allele frequency in 4-generation experiments (phase 2 of WHO guidelines)<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL # 6 Scaling up<\/td>\n\n
    \n
  • Production of large (10^9) quantities of GM embryos that can withstand transportation for several hours (12-24h) to be seeded in laboratories by a reservoir<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL #7 Pilot test<\/td>\n\n
    \n
  • Open field performance of GM mussels (phase 3 of WHO guidelines)<\/li>\n
  • Disinfestation of a reservoir branch upstream of a power plant using GM mussels in cages, in conjunction with chemical disinfestation of the plant<\/li>\n
  • Monitoring reinfestation and transgenic DNA using quantitative PCR (phase 4 of the WHO guidelines) .<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL #8 Regulatory stage<\/td>\n\n
    \n
  • Evaluation of the biosafety and efficacy of the genetic elements introduced<\/li>\n
  • Standard Operating Procedures for producing GM embryos, GM adults, planting seeds, GM mussels in cages, etc.<\/li>\n
  • Licenses, certificates and permits from MAPA (Ministry of Agriculture, Livestock and Supply), IBAMA, CTNBio (Brazilian Biosafety Technical Commission)<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL #9 Go to the market<\/td>\n\n