{"id":4445,"date":"2021-03-14T08:01:18","date_gmt":"2021-03-14T11:01:18","guid":{"rendered":"https:\/\/biobureau.com.br\/technological-readiness-scale-for-environmental-dna\/"},"modified":"2021-03-14T08:01:18","modified_gmt":"2021-03-14T11:01:18","slug":"technological-readiness-scale-for-environmental-dna","status":"publish","type":"post","link":"https:\/\/biobureau.com.br\/en\/technological-readiness-scale-for-environmental-dna\/","title":{"rendered":"Technological Readiness Scale for Environmental DNA"},"content":{"rendered":"

In 2020, the Bio Bureau began a research program to identify species using Environmental DNA, funded by Tijo\u00e1 Energia through ANEEL’s research and development program.<\/p>\n

We divided the development into stages according to the Technology Readiness Scale – TRL<\/a>. This division facilitates the understanding and communication of a project with a high degree of complexity.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Technology Readiness Level<\/td>\nTechnological goals<\/td>\n<\/tr>\n
TRL #1 Basic Principles<\/td>\n\n
    \n
  • Scientific papers supporting environmental DNA technology and metabarcoding as tools for routine environmental monitoring.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL #2 Conceptual model<\/td>\n\n
TRL #3 Proof of concept<\/td>\n\n
    \n
  • Validation in micro and mesocosms of the premises for sampling and analyzing biodiversity, with an emphasis on ichthyofauna, in hydroelectric reservoirs, using environmental DNA<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL #4 Lab-scale prototype<\/td>\n\n
    \n
  • Validation in macrocosms of the premises for sampling and analyzing biodiversity, with an emphasis on ichthyofauna, in hydroelectric reservoirs, using environmental DNA<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL #5 Field-scale prototype<\/td>\n\n
    \n
  • Initial characterization (baseline) of biodiversity estimated by environmental DNA in a hydroelectric reservoir. Establishment of a standard operating procedure for baseline estimation and biodiversity monitoring in hydroelectric reservoirs.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL # 6 Scaling up<\/td>\n\n
    \n
  • Adaptation of the protocol for application in different reservoirs, with validation in at least 3 reservoirs<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
TRL #7 Pilot test<\/td>\n\n
    \n
  • \n

    Methods and materials for scaling the technology, new diversity index based on DNA frequency, transactional business model<\/p>\n<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n

TRL #8 Regulatory stage<\/td>\n\n
    \n
  • \n

    Terms of reference, IBAMA and CONAMA technical notes<\/p>\n<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n

TRL #9 Go to the market<\/td>\n\n
    \n
  • National real-time monitoring program with 70% adherence of hydroelectric reservoirs<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    In 2020, the Bio Bureau began a research program to identify species using Environmental DNA, funded by Tijo\u00e1 Energia through ANEEL’s research and development program. We divided the development into stages according to the Technology Readiness Scale – TRL. This division facilitates the understanding and communication of a project with a high degree of complexity. […]<\/p>\n","protected":false},"author":2,"featured_media":4163,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[81],"tags":[],"_links":{"self":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts\/4445"}],"collection":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/comments?post=4445"}],"version-history":[{"count":0,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts\/4445\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/media\/4163"}],"wp:attachment":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/media?parent=4445"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/categories?post=4445"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/tags?post=4445"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}