{"id":4400,"date":"2020-11-20T16:41:55","date_gmt":"2020-11-20T19:41:55","guid":{"rendered":"https:\/\/biobureau.com.br\/theoretical-model-of-the-mussel-gene-drive\/"},"modified":"2025-06-26T11:29:24","modified_gmt":"2025-06-26T14:29:24","slug":"theoretical-model-of-the-mussel-gene-drive","status":"publish","type":"post","link":"https:\/\/biobureau.com.br\/en\/theoretical-model-of-the-mussel-gene-drive\/","title":{"rendered":"Theoretical model of the mussel gene-drive"},"content":{"rendered":"<p>To draw up the proposal, the Bio Bureau developed the theoretical model, which characterizes the 2nd stage on the <a href=\"https:\/\/app-64a79cc1c1ac1820c44e815f.closte.com\/2020\/11\/07\/fases-do-desenvolvimento-tecnologico\/\" rel=\"nofollow noopener\" target=\"_blank\">TRL scale<\/a>, based on the gene-drive for controlling the malaria vector (Anopheles gambiae) that was being developed by Andrea Crisanti&#8217;s group at Imperial College London.<\/p>\n<p>In this model, we would silence a mussel&#8217;s reproductive gene by inserting a (long) DNA fragment (construct) into the middle of its sequence, which encodes a set of enzymes capable of replicating this fragment and inserting it into the unmodified allele of the same reproductive gene on another chromosome.<\/p>\n<p>Self-replication of the construct and self-insertion into an unmodified copy allow this trait (infertility) to spread through the population at a faster rate than that expected by conventional Mendelian inheritance. This is why it is called super-Mendelian inheritance. Or Gene-Drive.<\/p>\n<p>The assumptions were validated with Andrea Crisanti himself, who is the project&#8217;s scientific advisor.<\/p>\n<p>The description of the model was formalized in an article published in pre-print format in 2018.<\/p>\n<ul>\n<li><span class=\"self-citation-authors\">Rebelo MF, Afonso LF, Americo JA, da Silva L, Neto JLB, Dondero F, Zhang Q.<\/span> <span class=\"self-citation-year\">2018<\/span>. <span class=\"self-citation-title\">A sustainable synthetic biology approach for the control of the invasive golden mussel (<i>Limnoperna fortunei<\/i>)<\/span> <span class=\"self-citation-journal\">PeerJ Preprints<\/span><span class=\"self-citation-elocation\">6:e27164v3<\/span> <a href=\"https:\/\/doi.org\/10.7287\/peerj.preprints.27164v3\" rel=\"nofollow noopener\" target=\"_blank\">https:\/\/doi.org\/10.7287\/peerj.preprints.27164v3<\/a><\/li>\n<\/ul>\n<p style=\"margin-left: 24pt; text-indent: -24.0pt;\">See also the article that inspired our project:<\/p>\n<ul>\n<li style=\"text-indent: -24pt;\"><span style=\"text-indent: -24pt; color: initial; font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif;\">Hammond, A., Galizi, R., Kyrou, K., Simoni, A., Siniscalchi, C., Katsanos, D., Gribble, M., Baker, D., Marois, E., Russell, S., Burt, A., Windbichler, N., Crisanti, A., &amp; Nolan, T. (2016). A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae.  <\/span><i style=\"text-indent: -24pt; color: initial; font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif;\">Nature Biotechnology<\/i><span style=\"text-indent: -24pt; color: initial; font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif;\">, <\/span><i style=\"text-indent: -24pt; color: initial; font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif;\">34<\/i><span style=\"text-indent: -24pt; color: initial; font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif;\">(1), 78-83. https:\/\/doi.org\/10.1038\/nbt.3439<\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>To draw up the proposal, the Bio Bureau developed the theoretical model, which characterizes the 2nd stage on the TRL scale, based on the gene-drive for controlling the malaria vector (Anopheles gambiae) that was being developed by Andrea Crisanti&#8217;s group at Imperial College London. In this model, we would silence a mussel&#8217;s reproductive gene by [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":4198,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[80],"tags":[93],"_links":{"self":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts\/4400"}],"collection":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/comments?post=4400"}],"version-history":[{"count":1,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts\/4400\/revisions"}],"predecessor-version":[{"id":5183,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts\/4400\/revisions\/5183"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/media\/4198"}],"wp:attachment":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/media?parent=4400"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/categories?post=4400"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/tags?post=4400"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}