{"id":4383,"date":"2020-11-21T08:01:23","date_gmt":"2020-11-21T11:01:23","guid":{"rendered":"https:\/\/biobureau.com.br\/validating-the-prototype\/"},"modified":"2025-06-26T11:26:59","modified_gmt":"2025-06-26T14:26:59","slug":"validating-the-prototype","status":"publish","type":"post","link":"https:\/\/biobureau.com.br\/en\/validating-the-prototype\/","title":{"rendered":"Validating the prototype"},"content":{"rendered":"<p><span style=\"font-weight: 400;\">With the validation of the prototype in a relevant environment, we have reached TRL# 5 and will end the experimental development stage &#8211; ANEEL&#8217;s DE. At this stage, we begin to evaluate the efficacy, efficiency and safety of the GMO.<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\"><span style=\"font-weight: 400;\">Laboratory experiments to advance the understanding of GMO safety, assessing the possibility of horizontal transmission (non-reproductive, unintentional, to non-target species) of the CRISPR construct, a prerequisite of the WHO (Phase I) and CTNBIO guidelines;  <\/span><\/li>\n<li style=\"font-weight: 400;\"><span style=\"font-weight: 400;\">Laboratory experiments to advance understanding of the efficacy of the GMO, assessing the frequency of vertical transmission (reproductive, intentional) over 5 generations, as suggested by the WHO guidelines (Phase I). Also evaluate the occurrence of mutations in GMO mussels.<\/span><\/li>\n<li>Experiments in <span style=\"font-weight: 400;\">micro and mesocosms (controlled environment, with containment, in the field) replicating the environment (same water and same species) of a reservoir, assessing by genotyping the possibility of horizontal transmission (non-reproductive, unintentional) of the CRISPR construct to other (cultivable) species in the aquatic trophic chain: bacteria, phyto- and zooplankton, other aquatic invertebrates and specifically molluscs, fish that eat mussels and fish that feed on other fish, as prerequisite of the WHO guidelines (Phase I and Phase II) and CTNBIO.<\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>With the validation of the prototype in a relevant environment, we have reached TRL# 5 and will end the experimental development stage &#8211; ANEEL&#8217;s DE. At this stage, we begin to evaluate the efficacy, efficiency and safety of the GMO. Laboratory experiments to advance the understanding of GMO safety, assessing the possibility of horizontal transmission [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":4116,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[80],"tags":[92],"_links":{"self":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts\/4383"}],"collection":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/comments?post=4383"}],"version-history":[{"count":1,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts\/4383\/revisions"}],"predecessor-version":[{"id":5177,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/posts\/4383\/revisions\/5177"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/media\/4116"}],"wp:attachment":[{"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/media?parent=4383"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/categories?post=4383"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biobureau.com.br\/en\/wp-json\/wp\/v2\/tags?post=4383"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}